High-Performance Liquid Chromatographic Determination of Oxytetracycline Residue in Cured Meat Products
نویسنده
چکیده
The development of a sensitive automated method for residue control of oxytetracycline (OTC) in cured meat (soudjouk) is described. The principle steps involve the extraction of OTC from cured meat in the presence of citric acid with nitric acid, methanol and de-ionized water, and then the determination by HPLC. In HPLC, Hypersil BDC C18 column was used and OTC was separated at 24◦C using a mobile phase of H2O (pH=2.10 with H2SO4): ACN (85:15, v/v) at a flow rate of 1.5 ml/min. A variable wavelength detector was set at 360 nm. The detection limit of the method was calculated at 8 ng/g and
منابع مشابه
Detection of Oxytetracycline Residue in Infant Formula by High-Performance Liquid Chromatography (HPLC)
Determination of drug residues in food is of great importance due to their toxicity. Long-term exposure with low level of drug residues could be important, especially in children. Based on document study, oxytetracycline (OTC) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause alle...
متن کاملDetection of Oxytetracycline Residue in Infant Formula by High-Performance Liquid Chromatography (HPLC)
Determination of drug residues in food is of great importance due to their toxicity. Long-term exposure with low level of drug residues could be important, especially in children. Based on document study, oxytetracycline (OTC) is a prophylaxis and treatment agent for great number of diseases and possesses a broad spectrum activity against many pathogenic organisms and can be toxic or cause alle...
متن کاملACROSS-SECTIONAL STUDY ON OXYTETRACYCLINE AND TETRACYCLINE RESIDUES IN PASTEURIZED MILK SUPPLIED IN TEHRAN BY AN HPLC METHOD
Tetracyclines (TCs) are broad-spectrum antibiotics that are used widely in veterinary medicine. The present study was carried out to trace the residues of oxytetracycline (OTC) and tetracycline (TC) in pasteurized milk that is marketed in Tehran with the use of a High Performance Liquid Chromatography (HPLC). Ninety milk samples were collected during five sequential days from the products of si...
متن کاملDetermination of oxytetracycline residues in cattle meat marketed in the Kilosa district, Tanzania.
Oxytetracycline is used to treat various diseases in cattle. However, its use may be associated with unacceptable residue levels in food. Oxytetracycline residues in tissues from indigenous cattle were determined in a cross-sectional study conducted in the Kilosa district, Tanzania, between November 2012 and April 2013. A total of 60 tissue samples, including muscle, liver and kidney, were coll...
متن کاملSimple High-Performance Liquid Chromatographic Method for Determination of Ciprofloxacin in Human Plasma
A rapid, simple and sensitive high-performance liquid chromatography method was developed for determination of ciprofloxacin in plasma by means of ultraviolet detection. Ofloxacin was used as an internal standard and separation carried on a Novapak C18 column using a mobile phase of 0.01 M phosphate buffer (pH =2.6): methanol (82: 18 v/v). Extraction of drug was performed from plasma by liquid-...
متن کامل